Abstract

Objective: To investigate the effect of t (11; 19) (q23; p13.1) gene on proliferation and apoptosis 0f SGC 7901 gastric cancer cell line Methods: Gastric cancer cell line SGC 7901 cells were selected to transfect t (11; 19) (q23; p13.1) gene. MRNA levels of t (11; 19) (q23; p13.1) in each group were regulated after 24, 48 and 72h byRT- PCR. Cell proliferation was determined MTT assay. The apoptosis status of the SGC 7901 cells was detected TUNEL method. Immunohistochemical staining evaluated the expression of apoptotic genes Bcl-2 and Bax. Results: The MTT assay showed that t (11; 19) (q23; p13.1) decreased the proliferation of SGC 7901 cells. TUNEL method detected t (11; 19) (q23; p13.1) could improve apoptosis of SGC 7901 cells. In addition, t (11; 19) (q23; p13.1) could improve the expression of apoptotic gene Bcl-2 and reduce the expression of apoptotic gene Bax. Conclusion: T (11; 19) (q23; p13.1) gene can inhibit gastric cancer cell proliferation and improve apoptosis of gastric cancer cell.

Keywords: T（11; 19）（q23; p13.1）gene；Cell proliferation；Cell apoptosis

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How to Cite
Zhou, H.-B., Ma, W.-Q., & Shao, L.-M. (2018). Effect of t (11; 19) (q23; p13.1) Gene on Proliferation and Apoptosis of SGC 7901 Gastric Cancer Cell Line. International Journal of Innovative Research in Medical Science, 3(12), 2367 to 2371. https://doi.org/10.23958/ijirms/vol03-i12/507

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Copyright © 2018 Hai-Bo Zhou Wang-Qian Ma Li-Ming Shao this is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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